. 2018 Dec 1;410:83-95.
doi: 10.1016/j.tox.2018.09.006. Epub 2018 Sep 13.
Graphene oxide nano-bio interaction induces inhibition of spermatogenesis and disturbance of fatty acid metabolism in the nematode Caenorhabditis elegans
- PMID: 30218681
- DOI: 10.1016/j.tox.2018.09.006
Graphene oxide (GO) has the potential for wide applications, which necessitates an intensive investigation of its potential hazard on human and environmental health. Even if previous studies show reproductive toxicity in the nematode Caenorhabditis elegans, the mechanisms of reproductive toxicity by GO are poorly understood. To understand the underlying mechanisms of GO-induced reproductive toxicity, we investigated the interaction between GO and C. elegans using Raman spectroscopy, sperm counts produced by spermatogenesis, progeny and analyzed the fatty acid metabolism using molecular techniques. GO-characteristic Raman spectral bands measured throughout C. elegans, brood size and Hoecst staining of dissected gonads clearly showed GO accumulation in the reproductive organs, reduced progeny and low sperm counts, which are possibly direct results of the reproductive toxicity from GO exposure. Interestingly, reduced fatty acid metabolites, such as stearic, oleic, palmitoleic, and palmitic acids, were found with GO exposure. We found that GO increased intestinal fat accumulation in wild type N2, fat-5(tm420), and fat-7(wa36) mutants, whereas it decreased fat storage in the fat-6(tm331) and nhr-49(nr2041) mutants. GO exposure affected C. elegans fat accumulation and consumption, which was possibly regulated by daf-16 and nhr-80 gene activity. Also, GO exposure suppressed the survival of long-lived fat-5(tm420) mutants, whereas it increased the survival of short-lived nhr-49(nr2041) mutants. Hence, our studies collectively indicated that GO accumulation in reproductive organs, suppression of spermatogenesis, and the alteration of fatty acid metabolism play critical roles in understanding mechanisms of toxicity in C. elegans.
Keywords: Caenorhabditis elegans; Fatty acid metabolism; Graphene oxide; Nano-bio interaction; Raman spectroscopy; Reproductive toxicity.
Copyright © 2018 Elsevier B.V. All rights reserved.
A systems toxicology approach reveals the Wnt-MAPK crosstalk pathway mediated reproductive failure in Caenorhabditis elegans exposed to graphene oxide (GO) but not to reduced graphene oxide (rGO).Nanotoxicology. 2017 Feb;11(1):76-86. doi: 10.1080/17435390.2016.1267273. Epub 2016 Dec 20.PMID: 27901397
Long-term exposure to thiolated graphene oxide in the range of μg/L induces toxicity in nematode Caenorhabditis elegans.Sci Total Environ. 2018 Mar;616-617:29-37. doi: 10.1016/j.scitotenv.2017.10.307. Epub 2017 Nov 3.PMID: 29107776
Intestinal Insulin Signaling Encodes Two Different Molecular Mechanisms for the Shortened Longevity Induced by Graphene Oxide in Caenorhabditis elegans.Sci Rep. 2016 Apr 4;6:24024. doi: 10.1038/srep24024.PMID: 27040644 Free PMC article.
Spermatogenesis-defective (spe) mutants of the nematode Caenorhabditis elegans provide clues to solve the puzzle of male germline functions during reproduction.Dev Dyn. 2010 May;239(5):1502-14. doi: 10.1002/dvdy.22271.PMID: 20419782 Free PMC article. Review.
Alternate metabolism during the dauer stage of the nematode Caenorhabditis elegans.Exp Gerontol. 2005 Nov;40(11):850-6. doi: 10.1016/j.exger.2005.09.006. Epub 2005 Oct 10.PMID: 16221538 Review.
Cited by 6 articles
Sex-reversal and Histopathological Assessment of Potential Endocrine-Disrupting Effects of Graphene Oxide on Japanese medaka (Oryzias latipes) Larvae.Chemosphere. 2021 Sep;279:130768. doi: 10.1016/j.chemosphere.2021.130768. Epub 2021 May 17.PMID: 34134430
Graphene and Reproduction: A Love-Hate Relationship.Nanomaterials (Basel). 2021 Feb 22;11(2):547. doi: 10.3390/nano11020547.PMID: 33671591 Free PMC article. Review.
Secoisolariciresinol Diglucoside Delays the Progression of Aging-Related Diseases and Extends the Lifespan of Caenorhabditis elegans via DAF-16 and HSF-1.Oxid Med Cell Longev. 2020 Jul 14;2020:1293935. doi: 10.1155/2020/1293935. eCollection 2020.PMID: 32733632 Free PMC article.
Novel Polymeric Nanocarriers Reduced Zinc and Doxycycline Toxicity in the Nematode Caenorhabditis elegans.Antioxidants (Basel). 2019 Nov 14;8(11):550. doi: 10.3390/antiox8110550.PMID: 31739428 Free PMC article.
Graphene-Based Nanomaterials in Soil: Ecotoxicity Assessment Using Enchytraeus crypticus Reduced Full Life Cycle.Nanomaterials (Basel). 2019 Jun 5;9(6):858. doi: 10.3390/nano9060858.PMID: 31195669 Free PMC article.
LinkOut – more resources
Full Text Sources
Other Literature Sources